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<br />City of San Marcos <br /> <br />TPDES Permit No. WQOO I 0273002 <br /> <br />conducting the TRE, including toxicity characterizations, identifications, confinuations, source <br />eyaluations, treatability studies, and/or alternative approaches. When conducting characterization <br />analyses, the permittee shall perform multiple characterizations and follow the procedures specified in <br />the document entitled, "Toxicity Identification Eyaluation: Characterization of Chronically Toxic <br />Effluents, Phase I" (EPN600/6-91/005F), or alternate procedures. The permittee shall perform multiple <br />identifications and follow the methods specified in the documents entitled, "Methods for Aquatic <br />Toxicity Identification Evaluations, Phase II Toxicity Identification Procedures for Samples Exhibiting <br />Acute and Chronic Toxicity" (EPN6001R-92/080) and "Methods for Aquatic Toxicity Identification <br />Evaluations, Phase ill Toxicity Confirmation Procedures for Samples Exhibiting Acute and Chronic <br />Toxicity" (EPN6001R-92/081). All characterization, identification, and confirmation tests shall be <br />conducted in an orderly and logical progression; <br /> <br />2) Sampling Plan - The TRE Action Plan should describe sampling locations, methods, holding times, chain <br />of custody, and preservation teclmiques. The effiuent sample yolume collected for all tests shall be <br />adequate to perform the toxicity characterization! identification! confirmation procedures, and chemical- <br />specific analyses when the toxicity tests show significant lethality. Where the pennittee has identified <br />or suspects specific pollutant(s) and/or source(s) of effluent toxicity, the permittee shall conduct, <br />concurrent with toxicity testing, chemical-specific analyses for the identified and/or suspected <br />pol1utant(s) and/or source(s) of effluent toxicity; <br /> <br />3) Quality Assurance Plan - The TRE Action Plan should address record keeping and data evaluation, <br />calibration and standardization, baseline tests, system blanks, controls, duplicates, spikes, toxicity <br />persistence in the samples, randomization, reference toxicant control charts, as well as mechanisms to <br />detect artifactual toxicity; and <br /> <br />4) Project Organization - The TRE Action Plan should describe the project staff, project manager, <br />consulting engineering services (where applicable), consulting analytical and toxicological services, etc. <br /> <br />c. Within 30 days of submittal of the TRE Action Plan and Schedule, the permittee shall implement the TRE <br />with due diligence. <br /> <br />d. The permittee shall submit quarterly TRE Activities Reports concerning the progress of the TRE. The <br />quarterly reports are due on or before April 20th, July 20th, October 20th, and January 20th. The report shall <br />detail information regarding the TRE activities including: <br /> <br />I) results and interpretation of any chemical-specific analyses for the identified and/or suspected <br />pollutant(s) performed during the quarter; <br /> <br />2) results and interpretation of any characterization, identification, and confirmation tests performed during <br />the quarter; <br /> <br />3) any data and/or substantiating documentation which identifies the pollutant(s) and/or source(s) of <br />effluent toxicity; <br /> <br />4) results of any studies/evaluations concerning the treatability of the facility's effluent toxicity; <br /> <br />5) any data which identifies effluent toxicity control mechanisms that will reduce effluent toxicity to the <br />}eyel necessary to meet no significant lethality at the critical dilution; and <br /> <br />6) any changes to the initial TRE Plan and Schedule that are believed necessary as a result of the TRE <br />findings. <br /> <br />Page 30 <br />