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<br />City of San Marcos <br /> <br />TPDES Permit No. WQOOl0273002 <br /> <br />TABLE 1 (SHEET 4 OF 4) <br /> <br />BIOMONITORING REPORTING <br />FATHEAD MINNOW GROWTH AND SURVIVAL TEST <br /> <br />FATHEAD MINNOW SURVIVAL DATA <br /> <br />Effluent Percent Survival in Mean percent CV%* <br />Concentration replicate chambers survival <br />(%) A B C D E 24h 48h 7 day <br />0% <br />8% <br />11% <br />14% <br />19% <br />25% <br /> <br />* coefficient of variation = standard deviation x 100/mean <br /> <br />2. Dunnett's Procedure or Steel's Many-One Rank Test or Wilcoxon Rank Sum Test (with Bonferroni <br />adjustment) or t-test (with Bonferroni adjustment) as appropriate: <br /> <br />Is the mean survival at 7 days significantly less (p=0.05) than the control survival for the % effluent <br />corresponding to lethality? <br /> <br />CRITICAL DILUTION (19%): <br /> <br />YES <br /> <br />NO <br /> <br />3. Enter percent effluent corresponding to each NOEC\LOEC below: <br /> <br />a.) NOEC survival = <br /> <br />% effluent <br /> <br />b.) LOEC survival = <br /> <br />% effluent <br /> <br />c.) NOEC growth <br /> <br />% effluent <br /> <br />d.) LOEe growth <br /> <br />% effluent <br /> <br />Page 35 <br />