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<br />2. <br /> <br />Modification <br /> <br />a. <br /> <br />Acidified sample is combined with 125 mls. Freon <br />in a flask and stirred at high speed for fifteen <br />minutes <br /> <br />b. <br /> <br />A second separation step is added <br /> <br />B. <br /> <br />Phenols - Std. Methods, <br />Extraction Method} <br /> <br />15th ed., <br /> <br />Part 510 B <br /> <br />(Chloroform <br /> <br />C. <br /> <br />Surfactants <br /> <br />1. <br /> <br />Std. Methods, <br />Method) <br /> <br />15th <br /> <br />ed. , <br /> <br />Part 512 A (methylene <br /> <br />Blue <br /> <br />2. <br /> <br />Analytical Chemistry, 38, p. 791 (Crystal Violet Method <br />using Hach Kit) <br /> <br />D. <br /> <br />Petroleum Distillates - Qualification by use of appropriate <br />solubility tests and solubility references. <br /> <br />V. <br /> <br />Determination of Microbiological Constituents <br /> <br />A. Total Coliform - Std. Methods, 15th ed., Part 909 A <br /> (Membrane Filter Procedure) <br />B. Fecal Coliform - Std. Methods, 15th ed., Part 909 C <br /> (Membrane Filter Procedure) <br /> <br />-79- <br />